KMID : 1225720220140040379
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Allergy, Asthma & Immunology Research : AAIR 2022 Volume.14 No. 4 p.379 ~ p.392
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Evaluation of Allergenicity on a ¥ø-5 Gliadin-Deficient Cultivar in Wheat-Dependent Exercise-Induced Anaphylaxis
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Lee Jong-Sun
Kim Sung-Ryeol Park Jong-Han Park Kyung-Hee Jeong Kyoung-Yong Lee Jae-Hyun Kang Chon-Sik Kim Kyeong-Hoon Park Jung-Won
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Abstract
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Purpose: ¥ø-5 gliadin is the major allergen that causes wheat-dependent exercise-induced anaphylaxis (WDEIA). Recently, a missing mutant wheat cultivar at 1B chromosome Glu-B3 and closely linked Gli-B1 loci was bred. This cultivar (¥ø5D) has a deficiency in ¥ø-5 and ¥ã-gliadins as well as some low-molecular-weight glutenins. We evaluated specific immunoglobulin E (sIgE) reactivity of the ¥ø5D in WDEIA patients compared to wild-type cultivar.
Methods: Serum samples from 14 WDEIA and 7 classic wheat allergy patients were used to compare the allergenicity of ¥ø5D and wild-type cultivars using immunoglobulin E immunoblotting, enzyme-linked immunosorbent assay (ELISA), and ImmunoCAP inhibition assays.
Results: Immunoblotting revealed that ¥ø5D extracts had less sIgE binding to gliadins and glutenins in WDEIA sera than wild-type extracts. Immunoblot inhibition assay for gliadin sIgE reactivity also showed that ¥ø5D gliadins had less allergenicity than wild-type gliadins. ELISA inhibition assay showed stronger allergenicity of gliadins than glutenins, although they had cross-reactivity. This assay also showed that the 50% inhibitory concentrations (IC50) of ¥ø5D extracts against gliadin- or glutenin-sIgE reactivity were approximately 4-fold higher in WDEIA patients than those of wild-type extracts. The inhibition capacity of ¥ø5D gliadins against recombinant ¥ø-5 gliadin-sIgE reactivity was also lower in WDEIA patients than that of wild-type.
Conclusions: The allergenicity of the ¥ø5D cultivar is markedly lower for WDEIA patients in the sIgE inhibition tests. These results suggest that the ¥ø5D cultivar may be a safe alternative for WDEIA patients.
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KEYWORD
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Food allergy, wheat, food-dependent exercise-induced anaphylaxis, gliadin, glutenin
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